The bacterial reverse mutation test uses amino-acid requiring strains of Salmonella typhimurium and Escherichia coli to detect point mutations by base substitutions or frameshifts. It detects mutations which revert mutations present in the test strains and restores the functional capability of the bacteria to synthesize an essential amino acid.
| Test model | Salmonella typhimurium TA 98, TA 100, TA1535, TA1537, TA97, TA97a, TA102, and E.coli WP2 UvrA |
| Assay Controls | Negative control: Aqueous and organic solvents Positive control: Strain specific positive control |
| Exposure time | 48 – 72 hours |
| Test item quantity | 2.0 grams |
| End point | Growth of revertant colonies |
| Data delivery | Mean number of revertant colonies per plate • 2-fold increase with vehicle control (TA 98, TA 100, TA97, TA97a, TA102, and WP2 UvrA) • 3-fold increase with vehicle control (TA 1535 and TA 1537) |
| Timeline | 4 – 5 Weeks |
